Journal: Frontiers in Cell and Developmental Biology

Article Title: Bone marrow stromal cells in Modic type 1 changes promote neurite outgrowth

doi: 10.3389/fcell.2023.1286280

Figure Lengend Snippet: MC1 compared to intra-patient control BMSC neurotrophic gene expression. (A) Gene set enrichment analysis (GSEA) of BMSC bulk RNA sequencing indicated enrichment of BDNF signaling-related pathways in MC1 BMSCs compared to control. (B) Gene expression of neurotrophic cytokines and respective receptors ( n = 6 MC1 + 6 intra-patient controls). Significance was tested using the paired t -test of MC1 compared to control log 2 fold change values and presented as log 2 fold change of MC1 vs. control BMSCs gene expression ± standard deviation.

Article Snippet: Gene set enrichment analysis (GSEA) was performed using GSEA software v.4.3.2 (University of California San Diego, CA, United States and Broad Institute, Cambridge, MA, United States) with a signal-to-noise ratio as the gene ranking metric.

Techniques: Control, Gene Expression, RNA Sequencing, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: Bone marrow stromal cells in Modic type 1 changes promote neurite outgrowth

doi: 10.3389/fcell.2023.1286280

Figure Lengend Snippet: Bulk RNA sequencing of SH-SY5Y cells co-cultured with MC1 or control BMSCs for 8 days. (A) SEMA3A is the most dysregulated gene detected and shown as transcript per millions (log 2 ratio = −0.339, p = 0.00, FDR = 0.043). (B) GSEA of SH-SY5Y transcriptome on the Reactome database of all terms with FDR < 25%. Results are shown as values of normalized enrichment score (NES).

Article Snippet: Gene set enrichment analysis (GSEA) was performed using GSEA software v.4.3.2 (University of California San Diego, CA, United States and Broad Institute, Cambridge, MA, United States) with a signal-to-noise ratio as the gene ranking metric.

Techniques: RNA Sequencing, Cell Culture, Control